Under the direction of a Principal Investigator, further develops and expands understanding of scientific theory in his/her area of interest. Incumbent also learns and applies scientific laboratory techniques and gains experience in other areas of research that will lead to increased probability of success as an independent scientist. This is a training position with an expected duration of 1 – 3 years.  The Pulikkan Lab focuses on uncovering the mechanisms of leukemogenesis and identifying novel therapeutic targets in CEBPA mutant and inv(16) acute myeloid leukemia (AML). Recently, the lab developed the first conditional knock-in mouse model for CEBPA mutations in AML. Successful candidates will work on projects related to the therapeutic targeting of chromatin deregulation in...

Provides data analysis, integration, and development of new computational methods to analyze and integrate genome wide datasets from transcriptome, epigenomic, and other types of functional genomics-based approaches.

Performs laboratory assays and complex experiments to elucidate novel research questions. The Xian Lab studies how mitochondrial stress controls cellular immunology in autoimmune diseases, Alzheimer's disease and cancer. We address how the immune sensing of mitochondrial DNA licenses inflammation and autoimmunity. We integrate functional genomics and proteomics with cellular and molecular immunology techniques, to understand the crosstalk between dendritic cells, CD4 T cells and macrophages. Ideal candidates will have skills in animal handling, lab management skills, strong commnication, and basic cellular/molecular skills in genotyping, PCR, flow cytometry, ELISA, cell immunofluourescence, Western blotting, cell culture, etc.

Under the direction of the Manager, Therapeutic Apheresis, the Apheresis Technician is responsible for assisting the Therapeutic Services RN with the collection of cellular therapy product collections at BCW and Milwaukee area hospitals. This includes preparing donors and equipment for apheresis procedures and operating the apheresis equipment while monitoring the patient or donor throughout the collection procedure. The Apheresis Technician will also assist in the coordination of stem cell collection, including NMDP and research cellular therapy collections, in addition to coordinating and performing granulocyte donations.

Under the direction of a Principal Investigator, further develops and expands understanding of scientific theory in his/her area of interest. Incumbent also learns and applies scientific laboratory techniques and gains experience in other areas of research that will lead to increased probability of success as an independent scientist. This is a training position with an expected duration of 1 year.  This project is part of our NIH R01 grant focused on defining how endothelial Rap1 isoforms regulate calcium and nitric oxide signaling to support EC–cardiomyocyte communication during cardiac growth and stress. We use endothelial-specific knockout mice, calcium imaging, vascular physiology, and endothelial cell – cardiomyocyte co-culture models to uncover mechanisms by which Rap1...

Develops, improves, and implements laboratory techniques and performs complex experiments and/or tests to contribute to the timely and accurate completion of the research project(s). This project is part of our NIH R01 grant focused on defining how endothelial Rap1 isoforms regulate calcium and nitric oxide signaling to support EC–cardiomyocyte communication during cardiac growth and stress. We use endothelial-specific knockout mice, calcium imaging, vascular physiology, and endothelial cell – cardiomyocyte co-culture models to uncover mechanisms by which Rap1 dysfunction impairs coronary flow and promotes heart failure.

Develops, improves, and implements laboratory techniques and performs complex experiments and/or tests to contribute to the timely and accurate completion of the research project(s). The Xian Lab studies how mitochondrial stress controls cellular immunology in autoimmune diseases, Alzheimer's disease and cancer. We address how the immune sensing of mitochondrial DNA licenses inflammation and autoimmunity. We integrate functional genomics and proteomics with cellular and molecular immunology techniques, to understand the crosstalk between dendritic cells, CD4 T cells and macrophages. Ideal candidates will have skills in animal handling, lab management skills, strong commnication, and basic cellular/molecular skills in genotyping, PCR, flow cytometry, ELISA, cell immunofluourescence,...

Develops, improves, and implements laboratory techniques and performs complex experiments and/or tests to contribute to the timely and accurate completion of the research project(s). The Jarjour lab studies tissue-resident immunity to pathogens, including viruses, bacteria, and helminths. Tissue-resident T cells are an area of special emphasis. This position will play an important role in launching the laboratory with primary responsibilities for managing/genotyping the mouse colony and ordering, as well as assisting with experiments and other more minor responsibilities. While prior laboratory experience is required, training will be provided in the specific techniques required for the position. Ideal candidates will have experience with animal handling, techniques, and euthanasia;...

Under the direction of a Principal Investigator, further develops and expands understanding of scientific theory in his/her area of interest. Incumbent also learns and applies scientific laboratory techniques and gains experience in other areas of research that will lead to increased probability of success as an independent scientist. This is a training position with an expected duration of 1 – 3 years.  The Sundd Lab is an NIH-funded lab that studies the role of inflammasome, nucleic acid sensing and interferon signaling in neutrophils and platelets in the pathophysiology of vaso-occlusion, lung injury and sterile inflammation in Sickle Cell Disease. The prospective candidate will have the opportunity to learn state-of-the-art techniques such as Multi-Photon-Excitation intravital...